Antigen Proteome Microarrays & Immunoprofiling
The antigen proteome microarray and immunoprofiling platform partner’s high throughput technology expedites biomarker and vaccine target discovery by measuring antibody responses to thousands of proteins in a single assay. Protein expression is carried out on a proteome level allowing the entire proteome to be screened for antigen targets using a small volume of biological samples. The partner’s library contains over 60,000 expressible Open Reading Frame (ORF) clones representing more than 50 medically important infectious disease causing organisms and human proteins. These clones can be used for the manufacture of protein microarrays.
Applications of the technology:
- Discovery of vaccine antigen candidates and correlates of protection
- Identification of strain-specific or cross-strain protective epitope(s) by scanning full length antigen with overlapping peptides (e.g. 30-200 amino acids)
- Study adjuvants and vaccine formulation for immunization optimization
- Clinical trial monitoring: screen volunteers for pre-existing immunity; measure patient immune responses to correlate treatment protocol and observed efficacy; identify biomarkers of infection, disease severity and resolution or other outcomes
- Therapeutic antibody target identification and validation of specificity
- Profiling antibody responses to complete or partial pathogen proteomes or human autoimmune antibodies (IgG, IgM, IgA, IgE and subclasses IgG1-4)
- Monoclonal and polyclonal antibody target identification and specificity (off-target cross-reactivity)
- Full-service project management: statistical analysis plan, experimental design, QA/QC, wet lab, statistical/bioinformatics analysis and reporting
- Customized protein microarrays (e.g. multi-pathogen arrays, new species proteomes, core- or pan(species)-proteome arrays, focused arrays containing selected proteins from single or multiple related pathogens), antigen tiling arrays (e.g. overlapping 20-200 amino acid peptides spanning full length region of target antigens)
Biological sample types that can be used with this technology:
- Reactive antibody screening: serum, plasma, saliva, breast milk/colostrum, antibody in lymphocyte supernatant (ALS), fecal, CSF, bronchoalveolar lavage (BAL), lyophilized blood and nasal wash.
- Monoclonal antibodies, polyclonal antibodies and hybridoma supernatants
- Host samples include humans, non-human primates, mouse, rat, rabbit and ruminant animals; custom assays can be developed